Manual nuclei segmentations in near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of mouse nucleus accumbens (jrc_mus-nacc-3)

Annotation description: Dense segmentations of nuclei in jrc_mus-nacc-3 using Amira-Avizo (Thermo Fisher Scientific) and "Using Amira to manually segment organelles in vEM for machine learning V.3" protocol.Annotation protocol: Using Amira to manually segment organelles in vEM for machine learning V.3 (http://dx.doi.org/10.17504/protocols.io.bp2l61rb5vqe/v3)Software: Amira 3D 2021.1, Classic Segmentation WorkroomClasses annotated: NucleiSource (EM) dataset ID: jrc_mus-nacc-3Source (EM) voxel size (nm): 4 x 4 x 3.8 (X, Y, Z)Source (EM) data dimensions (µm): 13.4 x 21.0 x 2.9 (X, Y, Z)Source (EM) DOI: https://doi.org/10.25378/janelia.24222913Dataset URL: s3://janelia-cosem-datasets/jrc_mus-nacc-3/jrc_mus-nacc-3.zarr/recon-2/labels/inference/segmentations/nuc/Visualization website: https://openorganelle.janelia.org/datasets/jrc_mus-nacc-3