Molecular Mechanisms of Acanthamoeba castellanii Response to Different Sources of Oxidative Stress

Oxidative stress
is a biological principle affecting all life on
Earth and is also an important factor in the pathogen-host relationship.
The pathogenic free-living amoeba Acanthamoeba castellanii has several pathways to cope with reactive oxygen species and the
damage that they cause. In this study, we aimed to provide a comprehensive
analysis of the amoeba’s response to different sources of oxidative
stress. Using whole-cell proteomic analysis, we obtained a complex
picture of the changes in the proteome and identified potential key
players in the defense against oxidative stress. Importantly, from
the differential proteomics analysis, we identified a candidate efflux
pump that may be involved in Acanthamoeba drug resistance.