Gene expression changes in mouse neuroepithelial organoids in response to all-trans retinoic acid.

We describe the transcriptomic changes in neuroepithelial organoids in response to all-trans retinoic acid. Organoids were grown via clonal growth from R1 mouse ES cells seeded in Matrigel and cultured in N2B27 medium at 37C, 5% CO2 as described (Meinhardt et al. Stem Cell Reports 2014, Ishihara et al. Methods in Mol. Bio. 2017). At Day 2 (=48 hrs post seeding), organoids were treated with or without 250 nM all trans retinoic acid. Organoids were extracted from the Matrigel at Day 2 or at 8, 18, 30, 42, 54, 66, and 78 hrs post Day 2. Three biological replicates were prepared for each cell population, yielding 45 independent samples for RNA extraction.