Transcript levels for β-catenin and Wnt10b during treatment with 1 µM Rosi.

U-33/γ2 cells were seeded on 6-well plates at the 1×104 cells/cm2 density in triplicates and 24 h later media was changed to either 1 µM Rosi supplemented or, as a vehicle, DMSO supplemented. RNA was isolated at the indicated time points from the beginning of treatment. The expression of β-catenin and Wnt10b after Rosi treatment were calculated as fold change as compared to the levels of these transcripts in a parallel culture of vehicle treated cells at the same time points. NC indicates that the tested gene expression in Rosi-treated cells was not significantly different from the expression of this gene in vehicle-treated control. Numbers indicate significant (pvs. vehicle-treated cells.