Single-nucleus sequencing data from developing and adult mouse olivocochlear neurons. Single-nucleus sequencing data from developing and adult mouse olivocochlear neurons

Transcriptional diversity of mouse olivocochlear neurons (OCNs) was examined using single-nucleus sequencing of brainstem cholinergic neurons at P1, P5, and P26-P28. This dataset includes multiple brainstem cell types, including OCNs and several other populations of cranial motor neurons. Overall design: Cholinergic nuclei were labeled using ChAT-Cre to drive expression of the nuclear localized Rosa26-Sun1-GFP reporter. GFP+ cells from the ventral brainstem were selected via fluorescence-activated cell sorting (FACS) and nuclei were captured using the 10x genomics single-cell 3' platform.