Cleavage factor II (CF II) is a poorly characterized component of the multi-protein complex catalyzing 3' cleavage and polyadenylation of mammalian mRNA precursors. We have reconstituted CF II as a he
The experiments were conducted to examine the effects of 22-bulge of pre-miRNAs on DICER cleavage in human cells. Overall design: HCT116 cells were transfected with pCDNA3 plasmids expressing pri-miRN
