Mass-spectrometric analysis of IDN2-6xMyc affinity purification.

The protein extracts were prepared from the IDN2-6xMyc transgenic plants in the wild-type and ros1idn2-4 mutant backgrounds and used for affinity purification of the IDN2-6xMyc-containing complex. Either total purified proteins or the ∼80-KDa band were subjected to mass-spectrometric analysis. Mascot score of each protein is shown. 1) The number is the total number of mass spectra matched to the corresponding protein, including the redundant ones that match to the same peptides. 2) The number is deduced from the “Matched queries” by removing the redundant peptides. 3) The number is calculated from “Matched queries” by removing the overlapped peptide sequences among its homologous protein family.