Single-cell RNA sequencing reveals a novel inhibitory effect of ApoA4 on NAFL mediated by liver-specific subsets of myeloid cells

The liver immune microenvironment is a key element in the development of hepatic inflammation in NAFLD. However, the precise immune cell subsets that exacerbate fatty liver remain elusive. ApoA4 deficiency increases the hepatic lipid burden, insulin resistance, and metabolic inflammation. The aim of this study was to profile the hepatic immune cells affected by ApoA4 in NAFL. We performed scRNA-seq on liver immune cells from WT and ApoA4-deficient mice administered a high-fat diet. Overall design: Liver immune cells isolated from WT and KO mice (n=3/group) were subjected to scRNA-seq