Evaluation of rsmA gene-delated strains by proteomic analyses

Normalized Spectral Abundance Factor (NSAF) data from three independent biological replicates were processed and stadistically evaluated to determine significant differences betwenn strains. Proteome analyses were performed in RStudio and included the evaluation of normality and homoscedasticity to assess the distribution of the NSAF data for triplicates of each identified protein. Differentially Abundant Proteins (DAPs) were identified based on the statistically significant differences in the NSAF values between conditions, defined by a Log2 Fold Change > 1.0 and p < 0.05 (determined using an unpaired t-test or Mann-Whitney U test, as appropriate). Additionally, proteins exclusively detected in the rsmA mutant strain were labeled in the tables as ON. In contrast, those identified only in the corresponding wild-type strain were labeled as OFF, suggesting negative and positive regulation by RsmA, respectively.