Kinetic parameters a for cytochrome c reductase with pea ferredoxin, E. coli flavodoxin, and E. coli ferredoxin, and the melting temperatures of thermal unfolding transitions for Xac-FNR, pea-FNR and Ec-FNR b.

aEach value represents the average of three independent determinations. A description of the calculation methods that were employed and the activity determinations are reported in the Materials and Methods. The original data are depicted in Figure S3.
bCytochrome c reduction was determined at 550 nm (ε550 = 19 mM−1 cm−1) as described in the Materials and Methods.
cTM is the temperature of the midpoint of the thermal denaturation transition and was determined as described in the Materials and Methods.
dAn estimate of the limit of the determination based on the tested sensitivity of the method.
eNot determined.
fValues of parameters for pea-FNR and Ec-FNR were obtained from a reference [49].