Single-cell RNA-seq analysis of Chuxiang black pigs and Yorkshire pigs

We obtained muscle tissue from the longissimus dorsi muscle of two Large White pigs and four Chuxiang black pigs for single-cell RNA-seq analysis using microfluidic techniques. In this study, six boars were used and raised in the same environment with the same feeding conditions. The Longissimus dorsi form these pigs were collected from the same position of thoracolumbar junction. Single cells were captured on a microwell chip, and nucleic acid-modified beads were used to molecularly label mRNAs from different cells, which were then constructed into a high-throughput single-cell transcriptome library. The NovaSeq 6000 sequencer was used to sequencing.