siRNA knockdown in neonatal rat cardiac myocytes
收藏NIAID Data Ecosystem2026-04-25 收录
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Primary neonatal rat cardiac myocytes were isolated and subjected to siRNA mediated Yap knockdown Overall design: Primary cardiac myocytes were isolated from two-day old rats via neonatal heart dissociation kit (Miltenyi Biotec) according to the manufacturer's protocol followed by percoll gradient separation. Cells were transfected with siRNAs designed against a combination of Llgl1 and Llgl2, Yap, or universal negative control siRNAs. Transfected cells were collected 48 hours post transfection for RNAseq.
创建时间：
2020-08-31
