Novel culture conditions facilitating the induction of naïve pluripotency in human embryonic stem cells. Homo sapiens

We report a novel culture condition inducing naive pluripotency in hESCs in a rapid, robust and reproducible way. These naive hESCs were similar to mESCs exhibiting domed colony morphology, increased single survival, reduced doubling time, upregulation of naive pluripotency-specific genes, unbiased lineage-specific differentiation, hypomethylation, separate clustering profile from parental primed hESCs and were dependent on signalling pathways similar to naive mESCs. Overall design: Global DNA methylation analysis of naive hESCs and their parental primed hESC counterparts.