Gene expression profiling of Trop2+ and Trop2- luminal epithelial cells and stromal cells from young adult and old mouse prostates

We set out to determine if distinct prostate luminal epithelial cell-types exhibited age-related differences in their gene expression profiles. We used fluorescence activated cell sorting to isolate Trop2+ and Trop2- luminal cells and EpCAM- CD49f- stromal cells from dissociated preparations of prostate tissue taken from young adult (3 months) and old (24 months of age) mice. RNA was extracted from sorted cells to evaluate differences in gene expression. Overall design: RNA sequencing of Trop2+ and Trop2- luminal cells and stromal cells isolated from 3 month and 24 month old mouse prostates. Experiments were performed using biological triplicates for stromal cells and duplicates for luminal subsets. Sequencing was performed on Illumina HiSeq 3000.