The application of non-cleavable cross-linkers to complex samples in XL-MS workflows is limited by the n² problem, a quadratic expansion of the search space with increasing database size. Here, a pept
Protein hotspot residues are key sites that mediate protein-protein interactions. Accurate identification of these residues is essential for understanding the mechanism from protein to function and fo
(A) RT-PCR analysis of calpains 8 and 9 mRNA in the WT, Capn8+/−, Capn8−/−, Capn9+/−, and Capn9−/− gastric mucosa using primer pairs: calpain 8-5′/-3′ for calpain 8, calpain 9-5′/-3′ for calpain 9, an