COL2a1/COL1a1 ratio (differential index) in alginate and CaReS® scaffolds.

The data obtained from mRNA expression of COL1a1 and COL2a1 was utilized to calculate the ratio between COL2a1 and COL1a1 (COL2a1/COL1a1) to show the differential index between two collagen types. It was shown that the differential index was significantly increased in cIL-4 transfected cells as compared to non-transfected controls overall showing high expression of COL2a1. All the samples were stimulated with rcIL-1β (100 ng/ml) and rcTNFα (50 ng/ml) for 96 h. Data is presented as mean ± S.D.