pSHDY# shuttle plasmid backbone, version w/o mobA Y25F point mutation. Precursor backbone of pSHDY as described by Behle et al., 2019 (https://doi.org/10.1101/757948).
Lipid A, a potent endotoxin which can cause septic shock, anchors lipopolysaccharide (LPS) into the outer leaflet of the outer membrane of gram-negative bacteria. MsbB acylates (KDO)(2)-(lauroyl)-lipi
On the sequence of the primers is represented: the restriction site (underlined); the Shine Dalgarno (lower case letter); start or stop codon (bold) for the forward (F symbol in the primer name) or re