Global changes in Acinetobacter baumannii in response to Ciprofloxacin

Transcriptomic, proteomic and gene dosage response of the clinical A. baumannii strain AB5075-UW upon exposure to sub-inhibitory concentrations of ciprofloxacin. Transcriptomics and proteomics analysis revealed a hypothetical gene ABUW_0098, named here as Acinetobacter ciprofloxacin tolerance (aciT) gene as being important in Ciprofloxacin tolerance. Disruption of this gene resulted in higher susceptibility to ciprofloxacin, and complementation of the mutant with a cloned aciT gene restored ciprofloxacin tolerance to parental strain levels. Microscopy studies revealed, that aciT is essential for filamentation during ciprofloxacin stress in A. baumannii.