Multiplex single cell analysis of serotonergic neuron function in planarians reveals widespread effects in diverse cell types

We used scRNA-seq to examine serotonergic function in asexual planarians. We profiled 47,292 single cell transcriptomes of planarian of 3 different RNAi categories: gfp(RNAi), lhx1/5-1(RNAi) and pitx(RNAi). We detected changes in cell type frequency and gene expression patterns in planarian of different knock-down conditions, capturing regulatory programs of distinct cell types in response to loss of serotonergic function. Overall design: We obtained cell dissociations from adult populations of the asexual strain of Schmidtea mediterranea of serotonergic trascription factors (pitx and lhx1/5-1) RNAi conditions.