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Multi-dimensional transcriptome analysis of an intact hematopoietic organ for HSPC expansion [10x]

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NIAID Data Ecosystem2026-03-12 收录
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To investigate the peculiarity of CHT HSPCs, as well as the interaction of CHT niche and HSPCs at higher resolution, we performed scRNA-seq with zebrafish CHT. The 52 hpf transgenic embryos were cut tails for dissecting into single cell suspension with PI staining. The 50 thousand kdrl:mCherry+ cells and 50 thousand CD41:GFP+ cells plus 50 thousands double negative cells were sorted by FACS for 10X genomics.

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2021-04-28
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