A new Cu(II)-based coordination polymer: crystal structure and protective effect on cardiac arrest induced by anesthesia via inducing SIRT1 signaling pathway

Based on a mixed-ligand strategy, a new coordination polymer with the chemical formula of [Cu(L)(1,4-NDC)]_n has been successfully synthesized by the reaction of L = 3,6-bis(benzimidazol-1-yl)pyridazine (L) and Cu(NO₃)₂·3H₂O with the presence of H₂NDC = naphthalene-1,4-dicarboxylic acid as the co-ligand under the solvothermal methods. Furthermore, a green hand grinding technique has been implemented to reduce the particle size of the complex <b>1</b> to generate nanoscale <b>1</b> (denoted as nano <b>1</b> hereafter) with spherical morphology. Furthermore, the protective activity of nano <b>1</b> on cardiac arrest induced by anesthesia was evaluated <i>in vivo</i>. Firstly, after the construction of the cardiac arrest mice model and compound construction, the left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) was measured to evaluate the cardiac functions. Then, the <i>sirt 1</i> and <i>nf-κb</i> genes expression level was detected by reverse transcription-polymerase chain reaction (RT-PCR) assay.