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Methanol-free Pichia pastoris for protein expression

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NIAID Data Ecosystem2026-03-10 收录
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The use of Pichia pastoris heterologous expression system is restricted due to limitations on the use of methanol. By deleting transcription repressors and overexpressing transcription activator of the aldehyde oxidase 1 (AOX1) promoter, a methanol-independent strain Δmig1Δmig2Δnrg1-Mit1 has been previously constructed. This study aimed to investigate the transcriptome and metabolome profile of the strain in response to different carbon sources.

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2018-02-10
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