ChIP-seq of human embryonic stem cells (hESCs) overexpressed with Monkeypox viral proteins H3L and A29L

We found that Monkeypox viral proteins H3L and A29L caused injuries in human cells. Whether they can bind chromatin and re-model chromation is unclear. Here, we applied ChIP-seq on hESCs to evaluate whether and how they are involved in chromatin regulation. Overall design: ChIP was conducted using anti-myc tag, anti-H3K4me4 or anti-H3K27me3 on human embryonic stem cells (hESCs) overexpressed with Monkeypox viral proteins H3L and A29L. Then chromatin DNAs were purifed for sequencing.