five

Human immunodeficiency virus 1 Raw sequence reads. Human immunodeficiency virus 1

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NIAID Data Ecosystem2026-03-14 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA936657
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To do so, we perform an in vitro primer elongation experiment with HIV polymerase on a 100mer RNA (modified or not on a single site) corresponding to an HIV-1 genome sequence and a DNA primer. We test different reverse transcription conditions, mimicking different cell types infected by the virus (activated or quiescent cells with more or less dNTP for example). The reverse transcription products are then amplified by PCR and sequenced at the IHU platform.Translated with www.DeepL.com/Translator (free version)
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2023-02-20
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