Profiling of Polyadenylated RNAs after Brg1/Smarca4 knock-down in murine bone marrow-derived macrophages after LPS or LPS plus Dex stimulation [RNA-seq]

We performed Brg1 (Smarac4) knock-down by siRNA in murine primary bone marrow-derived macrophages and profiled the effect on gene expression by RNAseq after lipopolysaccharide (LPS) or LPS plus dexamethasone (Dex) treatment. Brg1 knock-down cells are compared to cells treated with a control siRNA. We analysed the requirement of Brg1/Smarca4 for the expression of inflammatory and glucocorticoid receptor target genes. We performed gene silencing with small interfering RNA (siRNA) against Brg1 in murine bone marrrow-derived macrophages and compare dthose cells to cell streated with a control siRNA (ctrl). Cells were treated with 50nM sicontrol or siBrg1 for 48 hours and in the last 6 hours with vehicle (0.1% EtoH, 0.1%D-PBS), LPS (100ng/ml LPS and 0.1% EtoH) or Dex+LPS (100ng/ml LPS and 1µM Dex). Three replicates were sequenced per condition and treatment.