CWC 2012-2014 Louisiana marsh sediment bacterial communities after the Deepwater Horizon oil spill

This study was done to evaluate changes in microbial communities from coastal marsh subtidal sediments, inland soils, and water in southern Louisiana. These marshes were impacted by the Gulf of Mexico oil spill in 2010, and this project is a continuation and extension of PRJNA232457 (from 2010 - 2013). New samples were acquired from core locations as part of the Coastal Waters Consortium, from June 2012 through October 2014. Bacterial communities were assessed from subtidal sediments collected at 1-2 m from the marsh vegetation edge or from soils collected 5 m inland from the edge (labeled with IN). Water samples were collected from the edge (labeled with EW). Sediment and soil were collected from push cores and sectioned in the field at (A) 0-1 cm, (B) 1-2 cm, (C) 4-5 cm, and (D) 9-10 cm depth intervals. From extracted DNA, the V1-V3 region of the bacterial 16S rRNA gene was amplified and single-direction reads were generated using multiplexed, bar-coded primers for pyrosequencing.