Overview of attempts to create ‘hexas’ for <i>TaPLDß1</i> and <i>TaWRKY11</i>.

Number of progeny screened (F2 progeny) and number of progeny identified with expected tetra and hexa genotypes (frequency of primary mutants is not shown; these were similar to expected for all crosses. ‘-’ indicates that a given parent was not used in the cross, or a given deletion was not expected in the progeny. N.B. for TaPLDß1 A-D tetras were crossed with B-D tetras to produce hexas, and thus all progeny were expected to possess a D deletion. Therefore AB-deletion lines (possessing deletions of A and B, but with D intact) were not expected within the progeny. Asterisks indicate significantly different observed frequencies of a deletion genotype compared to expectations; * indicates P < 0.05; ** indicates P < 0.01. Where a deletion genotype was not identified and this was significantly different from expectations, this is in bold. ‡ ‘Index’ refers to the expected genotypic ratio within F2 progeny of crosses performed as given in Table E in S1 File. Overview of attempts to create ‘hexas’ for TaPLDß1 and TaWRKY11.