Transcriptomes of Fusarium verticillioides strains 7600 (wild type) and FV013 (HAP3 deletion mutant)

To elucidate genes regulated by the HAP complex in F. verticillioides, the transcriptomes of 7600 (wild type) and FV013 (HAP3 deletion mutant) grown for three days on minimal medium were sequenced. These conditions were selected for gene expression profiling based on pronounced differences in radial growth between the wild type and HAP3 deletion strain as well as the visible derepression of pigmentation in the HAP3 deletion strain compared to the wild type. F. verticillioides strains were center inoculated (5 µl of a 1 × 106 per ml conidial suspension) onto solid minimal medium overlaid with sterile cellophane discs and incubated at 23 °C in darkness. Three days after inoculation, the cellophane discs and mycelia were removed and ground in liquid nitrogen. Total RNA was isolated with Trizol reagent following the manufacturer’s recommendations. RNA-seq was performed on the Illumina Hi-Seq 2000 platform (Purdue University Genomics Core Facility, West Lafayette, Indiana). Libraries were prepared from RNA of F. verticillioides strains 7600 and FV013. Each RNA sample was derived from tissue pooled from ten cellophane overlays, and one pooled sample was sequenced per strain. The two libraries were indexed and sequenced in one lane with paired-end sequencing (100-bp reads).