Evaluation of metabolomic changes in serum and intestinal content of morbidly obese patients subjected to bariatric intervention - part 2 Bile acids analysis

<p class="MsoNormal">Obesity, a major health condition in the modern world, poses
numerous health risks and can reduce life expectancy by 8 to 10 years. The most
effective treatment for obesity remains bariatric surgery, which results in
significant weight loss and improved metabolism. Currently, few surgical
techniques are performed, and the choice of the procedure is dependent on the
age of the patient, the presence and severity of co-morbidities, and the BMI.
Some of the most commonly performed bariatric procedures are laparoscopic
sleeve gastrectomy (LSG) and one anastomosis gastric bypass (OAGB). Both
techniques are effective in inducing weight loss and improving markers of
obesity-related diseases, as well as overall quality of life. Although the
clinical outcomes and differences between bariatric procedures are well
established, data on procedure-specific metabolic alterations remain limited. Many
studies indicate an increase in circulating BA after various types of BS. This
is due to the changes in anatomical structure, but also as a result of an
altered intestinal microbiota. However, this effect is not fully understood in
the long term. Therefore, the aim of our study was to validate an analytical
method and apply it to the analysis of serum bile acids in female patients
after both LSG and OAGB. By including up to a 9–12-month follow-up and a group
of healthy, non-obese women as controls, we aimed to obtain a broader
understanding of the long-term metabolic changes occurring post-surgery.</p>