On-capillary Alkylation Micro-reactor: A facile strategy for proteo-metabolome profiling in the same single mouse oocytes

An on-capillary alkylation micro-reactor (OCAM) was developed for parallel measurement of proteome and metabolome in the same single mouse oocytes, enabling the identification of 1775 protein groups and 107 metabolites. By quantitative proteo-metabolome analysis of single oocytes at germinal vesicle (GV) stage, comprehensive characteristics of the different metabolic patterns of SN (surrounded nucleolus) and NSN (non-surrounded nucleolus) oocytes was revealed.