Trajectory analysis quantifies transcriptional plasticity during macrophage polarization

Ctyokine-stimulated polarization and repolarization of murine bone marrow-derived macrophages (LPS + IFNγ, IL-4); bulk RNA-seq timecourse and bulk ATAC-seq for selected timepoints and conditions Bulk RNA-seq and bulk ATAC-seq was performed on mouse bone marrow-derived macrophages treated with either LPS + IFNγ or IL-4 at multiple timepoints after cytokine-induced polarization and repolarization. Unpolarized macrophages were used as a control. Three biological replicates were performed for all bulk experiments; one biological replicate was performed for the 10X experiment.