Single cell RNA sequencing of Human Fetal Liver and Thymus samples

Single cell RNA TCR sequencing data reveal antigenic selection in T effector cells in peripheral liver tissue samples FACS sorting of CD45+CD3+CD14- T cells were performed with the BD FACS ARIA II system and FCS sorting data was collected via the BD FACSDiva software. Sort-enriched T cells were prepared for single-cell RNA-sequencing (scRNA-seq) using the Chromium Next GEM Single Cell 5ʹ v2 (Dual Index) protocol (10X Genomics) according to manufacturer’s recommendations. Single cells were encapsulated into droplets with a 8 gel bead in emulsion (GEMs) method using the 10X Chromium controller, where each mRNA transcript is tagged with a unique oligonucleotide barcode. Downstream library constructions were done using Library Construction kit (10X Genomics) with Chromium Single Cell Human TCR Amplification Kit (10X Genomics) and barcoded with i7 Illumina adapter indexes from Dual Index Kit TT Set A (10X Genomics). Pooled libraries are then sequenced on the Illumina HiSeq 4000 platform. *************************************************************** Raw files for human/patient samples were not submitted to GEO due to concerns about submitting personally identifiable sequence data for open access. ***************************************************************