Transcriptomics of Mouse Hepatitis Virus Strain A59 and nsp15 NTD mutants infected C57bl/6 Bone Marrow Derived Macrophages

The study aims to identify non-catalytic residues of Coronavirus nsp15 that regulate EndoU activity during viral replication and to globally evaluate how the loss of EndoU function results in activation of the host innate immune response and subsequent cell death. Total RNA from mock, MHV-A59- and nsp15 mutants-infected C57bl/6 mouse Bone Marrow Derived Macrophages (BMDMs) were extracted in triplicates at 3, 6, 9 and 12 hours post infection (with mock only at 3 hpi). Directional RNA sequencing was performed at the University of Chicago Genomics Facility on a Novaseq X.