The mitochondrial gene CMPK2 functions as a rheostat for macrophage homeostasis in inflammation

We report the impact of altered CMPK2 expression in THP1 macrophages. CMPK2 silencing THP1 macrophages (CM) were constructed by stably expressing siRNA against CMPK2. Scrambled siRNA expressing THP1 cells were used as control (SC). THP1 macrophages stably expressing CMPK2 fused with mCherry was used as CMPK2 overexpressing macrophages (OE). Control cells were constructed by overexpressing mCherry alone (VC). The RNA sequencing was used to identify genes that are differentially expressed in CMPK2 modified THP1 macrophages. Overall design: Identifing the effect of CMPK2 alteration in THP1 macrophage inflammatory response