Ag-specific IgG isotype responses in mice vaccinated with different vaccine regimens in long-term protected group (twelve weeks post boost) before and after 3 months L. donovani challenge infection.

Twelve weeks after final immunization and 3 months after challenge infection, blood serum samples were collected and assayed for IgG2a, IgG1, and IgG2a:IgG1 by ELISA. The results are shown as the mean absorbance values ± S.E. of five individual mice per group, representative of two independent experiments with similar results. p values were calculated using one-way ANOVA and Tukey's multiple comparison test.aSignificantly higher than gp63 DNA either free or in presence of CpG (pbSignificantly higher than gp63 DNA either alone or in association with CpG, and rgp63 plus CpG (pcSignificantly higher than DNA-prime/Protein-boost (p