An instructive role for IL7RA in the development of human B-cell precursor leukemia

10X single cell analysis of human leukemia, pre-leukemia and normal control. The leukemia was developed in xenograft mice that was serialy transplanted with cells from primary mouse that was transplanted with normal cord blood after transduction with virues encoding hyper activated mutant IL7RA. The preleukemic cells are from the primary engrafted mouse and the control is the same cord blood that was transduced with backbone virus expressing GFP. human CD10+CD19+ cells from the BM of the leukemic mouse (Leukemic F49), the pre-leukemic mouse (Preleukemic 49_Q2) and the mouse engrafted with matching CB transduced with control BackBone virus (Backbone BB) were sorted. Additionally, the preleukemic mouse sample separately sorted the CD10highCD19low sub-population (preleukemic 1, 49_P2). Cells were subjected to 10X single cell gene expression analysis