Quartet RNA reference datasets

As part of the Quartet Project for quality control and data integration of multiomics profiling, we establish four RNA reference materials derived from immortalized B-lymphoblastoid cell lines from four members of a monozygotic twin family. Additionally, we construct ratio-based transcriptome-wide datasets between two samples, providing cross-platform and cross-lab ‘ground truth’ references. Investigation of the intrinsic biological differences among the Quartet samples enabled sensitive assessment of cross-batch integration of transcriptomic measurements at the ratio level. The Quartet RNA reference materials combined with the ratio-based reference datasets can serve as unique resources for assessing and improving the quality of transcriptomic data in clinical and biological settings.