E14 cells knockdown with shRNA

Transcription factors are considered to be the master switches that determine the state and fate of cells. Sox2 is an important transcription factor necessary for the pluripotency and self-renewal of embryonic stem cells. Sox2 is generally considered to play a role in the initiation of transcription, but recent studies have shown that Sox2 may influence the alternative splicing of pluripotent genes with the assistance of proteins associated with RNA metabolism, suggesting that Sox2 may be regulated in a novel way during the maintenance of pluripotency in embryonic stem cells. Using mouse embryonic stem cell model, this project focused on Sox2 and proteins related to RNA metabolism to explore their mode of action and the mechanism of regulating the maintenance of pluripotency of embryonic stem cells. Overall design: Comparative gene expression profiling analysis of RNA-seq data for E14 cells and its KD derivatives (shCdk9, shHnrnpk, and shSox2).