Steady-state and time-resolved data for the article "Genetically encoded non-canonical amino acids reveal asynchronous dark reversion of chromophore, backbone and side-chains in EL222"

1. Steady-state data

Spectra were taken with/without continuous irradiation with a wavelength of 450 nm.

1.1 CNF absorption region (2200-2260 cm-1)

1.2. Amide absorption region (1500-1800 cm-1)

1.3. UV/Visible absorption region (320-550 nm)

Each spectral region has 3 datasets:

- DARK

- LIT (photostationary state)

- DIFFERENCE (lit-minus-dark)

The first row contains the variants.

The first column contains the wavenumber (in cm-1) or wavelength (in nm).

2. Time-resolved data.

Dark recovery reaction after switching lights off (wavelength of 450 nm).

2.1. EL222_WT_H2O

2.2. EL222_WT_D2O

2.3. EL222_W31CNF

2.4. EL222_L35CNF

2.5. EL222_N53CNF

2.6. EL222_Y136CNF

2.7. EL222_M151CNF

2.8. EL222_L216CNF

2.9. EL222_LOV_H2O

2.10. EL222_LOV_D2O

For each variant we have measured 3 spectral regions (except for 2.1, 2.2., 2.9 and 2.10, which lack CNF):

- CNF: 2200-2270 cm-1

- AMIDE: 1500-1750 cm-1

- UVVIS: 320-550 nm

For each probe we have 2 datasets:

- DATA: the experimental lit-minus-dark absorbance difference spectra. 

The first row contains the time delays (in seconds).

The first column contains the wavenumbers (in cm-1) or wavelength (in nm).

- LDA: the lifetime density maps calculated from the corresponding DATA using the inverse Laplace transform and maximum entropy penalty.

The first row contains the lifetimes (in seconds).

The first column contains the wavenumbers (in cm-1) or wavelength (in nm).

If you use these datasets please cite:

Chaudhari, A.S.; Chatterjee, A.; Domingos, C.A.O.: Andrikopoulos, P. C.; Liu, Y.; Andersson, I.; Schneider, B.; Lórenz-Fonfría, V.A.; Fuertes, G. Genetically Encoded Non-canonical Amino Acids Reveal Asynchronous Dark Reversion of Chromophore, Backbone and Side-chains in EL222. Protein Science. 2023 https://doi.org/10.1002/pro.4590