Dataset for "Development of a Triplex FMCA Assay for Genotyping Three Genes, ADH1B, ADH1C, and ALDH2, Involved in Alcohol Metabolism"

This dataset contains raw data and analysis files for the triplex FMCA assay targeting ADH1B, ADH1C, and ALDH2. Note the following orientation of the sequences and primers used in this study:Gene Orientations:ADH1B and ADH1C: These genes are located on the minus (reverse) strand of the reference genome.ALDH2: This gene is located on the plus (forward) strand.Sanger Sequencing and FMCA considerations:ADH1B: Sequences are analyzed in the reverse orientation (minus strand).ADH1C: Analysis was performed in the reverse orientation using a reverse primer.ALDH2: Analysis was performed in the forward orientation (plus strand).Raw sequencing data: Raw sequencing data (both .ab1 and .fasta formats) obtained from the bidirectional sequencing of ADH1B, ADH1C, and ALDH2 using specific forward and reverse PCR primers.This information is essential for interpreting the probe-target melting curves and the genotyping results provided in this dataset.