Quantified mutant phenotype values.

a“RPM area” is the area in square microns of the smallest bounding box required to enclose all positions of a spot in 60 successive time-lapse images made at 1 acquisition per second.
bMedian RPM area values for unpaired spots in units of µm2/minute. RPM area for wild-type at t0 is 0.28; each pairwise comparison with wild-type at t3 is significantly different at P<0.001 by Kolmogorov-Smirnov test, except for mps3-dCC where P = 0.64.
cMedian values of change in percent paired from t3 to t5, in units of percent paired per hour. By Mann-Whitney U test, the P values for the differences between wild-type and mutants are 0.066, 0.034 and 0.0025, respectively, and for mps3-dAR versus ndj1Δ is 0.025.
dMedian RPM area values for paired spots in units of µm2/minute. By Mann-Whitney U test, the P values for the differences between wild-type and the mutants are 0.22, 0.052, 3.6×10−5, 7.9×10−7, and 9.3×10−5, respectively.
eMedian RPM area values for unpaired spots in units of µm2/minute. By Mann-Whitney U test, the P values for the differences between wild-type and the mutants are 1.2×10−5, 3.7×10−16, 5.8×10−17, 2.1×10−31, and 1.4×10−26, respectively.
fViable spore production is estimated by multiplying the mean fractions of asci with 3 or 4 spores formed per sporulating cell by the mean number of viable spores in 4-spored asci, which provides a rough estimate of the number of viable spores produced per sporulating cell. By chi-square, wild-type sporulation (number of asci formed per sporulating cell) and spore viability (Figure 7A, 7B) each differs from all mutants by P<10−4.
gMean values of disomic colonies per total number of viable colonies. By t-test, wild-type is not significantly different from mps3-dCC (P = 0.21) but differs from mps3-dAR with P = 1.0×10−4 and P values are smaller for the other mutants.
hMean values of cells with premature sister chromatid separation of chromosome 7, as percent of population. By t-test, wild-type is not significantly different from mps3-dCC (P = 0.26) but differs from mps3-dAR with P = 0.016 and from the other mutants with P<0.01. For mps3-dNT versus csm4Δ, P = 0.075.
iFraction of population with telomere cluster and spindle-pole proximity values of less than 0.6, roughly corresponding to the cut-offs between bouquet and non-bouquet nuclei, when in the rec8Δ background. By chi-square, rec8Δ alone (in the “wild-type” column of the table) differs from each of the 3 double mutants with P<0.005; the 3 double mutants are not statistically significantly different from one another.
jFraction of population with telomere to spindle-pole proximity values of less than 0.6, roughly corresponding to the cut-off between bouquet and non-bouquet nuclei, when in rec8Δ background. By chi-square, rec8Δ alone (in the “wild-type” column of the table) differs from each of the 3 double mutants with P<0.02; the 3 double mutants are not statistically significantly different from one another.
kFraction of population with telomere cluster values of less than 0.6, roughly corresponding to the cut-off between bouquet and non-bouquet nuclei, when in rec8Δ background. By chi-square, rec8Δ alone (in the “wild-type” column of the table) differs from each of the 3 double mutants with P<10−7. The rec8Δ mps3-CC mutant shows more clustering than rec8Δ ndj1Δ (P = 0.0003); the rec8Δ mps3-dAR mutant shows less clustering than rec8Δ ndj1Δ (P = 0.05).
NA – Not available.