SEM and corresponding TEM micrographs of the epithelial and stromal layers in preserved AM substrates.

Fresh, (A, F); cryopreserved, (B, G); denuded (C, H); dried (D, I) and post treatment with trehalose (E, J). Micrographs depict extensive damage to the AEC layer and microvilli post cryopreservation compared to fresh, dried and trehalose treated substrates. Images shown are representative of triplicate experiments carried out on three donor membranes.