Copolarization of Gag with Cellular Markers.

Primary CD4+ T cells and P2 cells infected with VSV-G-pseudotyped HIV-1 encoding Gag-YFP were cultured for two days, immunostained for cellular proteins, and examined by fluorescence microscopy (see Materials and Methods). Gag-YFP-positive cells that were categorized as polarized cells based on cell morphology were further examined for polarized localization of Gag-YFP and copolarization of Gag-YFP with cellular markers. aIn P2 cells where Gag did not copolarize with PSGL-1, PSGL-1 did not show polarized localization. bIn P2 cells where Gag did not copolarize with MTOC, MTOC localized near the center of cells.