Predicted elemental biomass composition (bold) estimated from overall chemical reactions for growth on acetate.

The color of blue for the overall cellular reaction indicates that the cell operates in a reductive cycle, as shown in Fig 1. Estimates were obtained from simulations under oxidizing, neutral and reducing redox conditions, according to the concentrations of NADP+:NADPH being held at the thermodynamic odds (Eq 24) of 1010, 107 and 103, respectively. The rows labeled Simulation are those from the optimization for growth of the metabolic model while those labeled Estimate are estimates of the biomass elemental composition using the stoichiometry found in the model. The grey highlighted rows are for those compounds in the overall chemical equation for the metabolic model that are not used in estimating the elemental composition of the biomass. Abbreviations: ABP: adenine-3,5-bisphosphate; THF: tetrahydrofolate; 5,10-MTHF: 5, 10 methylenetetrahydrofolate; N10-fTHF: N10-formyltetrahydrofolate; Pi,2: diphosphate.