Genomic surveillance of vancomycin-resistant Enterococcus faecium reveals spread of a linear plasmid conferring a nutrient utilization advantage

Raw<b> </b>data<b> </b>for all growth curves of the paper<b> </b>and four additional figures<b><br></b><b><br></b><b>Figure legends</b>:<b> </b>Growth_M1_PM1: Growth in M1 supplemented with 95 single carbon sources (PM1 BioLog Inc). The mean of three biological replicates for each representative isolate is displayed, with the standard error of mean shaded. Conditions are ordered alphabetically, blank well (only M1) is also shown (second row, third column).<br>Growth_M1_PM2A: Growth in M1 supplemented with 95 single carbon sources (PM2A BioLog Inc). The mean of three biological replicates for each representative isolate is displayed, with the standard error of mean shaded. Conditions are ordered alphabetically, blank well (only M1) is also shown (second row, eleventh column).<br>Growth_M1_GalNAc: Growth in M1 medium and M1 medium supplemented with 0.1% GalNAc for the isolates of<i> Cluster 10</i> (<b>A</b>)<b> </b>and<i> Cluster 6 </i>(<b>B</b>). In <b>A</b>, the <i>ancestral</i> isolates are in orange and the <i>outbreak clade </i>isolates are in red, excluding the only clade member which did not carry pELF_USZ, which is in magenta. In <b>B</b>, the isolates which carried pELF_USZ are in purple (VRE32_P32 and VRE46_P41_01), while the others are in yellow (VRE30_P30, VRE36_P36, VRE39_P10_02, VRE47_P41_02, VRE49_P41_03). <i><br></i><br>Growth_BHI: Growth curves based on optical density measurements (OD₆₀₀) every 10min during 24h for the isolates of the persistent clone (<b>A</b>);<b> </b>and the recipient and transconjugant (<b>B</b>). The average of three biological replicates is shown. Standard error of mean is shown with a shaded area in <b>B</b> and omitted in <b>A</b> for better visibility. <i> </i>