John E. Michaels, Patricia A. Brown (2010) CIL:10054, Rana catesbeiana, barrier epithelial cell. CIL. Dataset

This group of micrographs illustrate the ultrastructural characteristics of the middle layer of the three meningeal layers that cover the central nervous system. The arachnoid cells contain abundant intermediate filaments that occupy a substantial volume of the cytoplasm. In some cells the intermediate filaments are organized into numerous coils of relatively uniform size and orientation, and some coils are closely associated with the nuclear envelope, as is shown in this micrograph. The coils appear to stack in loose hexagonal arrays (see CIL:9301). The plasma membrane of these cells has many caveolae. Adjacent arachnoid cells are connected by large desmosomes. Images were recorded using a Philips EM301 TEM at 80 kv. Original glass plates were scanned and digitized using an Epson V750 flatbed scanner with a step size of 15 micrometers. This group of images complement those published in the reference below. Reference: Michaels, JE, Tornheim, PA. Cell Tissue Res. 236:693-697 (1984).

本组显微照片展示了覆盖中枢神经系统的三层脑膜中层结构的超微结构特征。蛛网膜细胞富含中间丝，这些中间丝占据了细胞质相当大的体积。在某些细胞中，中间丝组织成数量众多、尺寸和方向相对均匀的螺旋状结构，其中一些螺旋状结构与核被膜紧密相连，如图所示。这些螺旋状结构似乎以松散的六边形阵列形式堆叠（参见CIL:9301）。这些细胞的质膜上有许多小窝。相邻的蛛网膜细胞通过大型桥粒连接。图像使用Philips EM301透射电子显微镜在80 kv的电压下记录。原始玻璃板使用Epson V750平板扫描仪，步进距为15微米进行扫描和数字化。本组图像补充了以下参考文献中发表的内容。参考文献：Michaels, JE，Tornheim, PA. 细胞与组织研究，第236卷，第693-697页（1984年）。