The transcriptome analysis in ppsnrk2 quadruple knockout

The plant-specific SNF1-related protein kinase 2 (SnRK2) family is a key regulator of osmostress and abscisic acid (ABA) responses in angiosperms. Osmostress-responsive SnRK2s can be classified into three subclasses (I-III), and the subclass III SnRK2s arose first in plants as the prototype, which is an essential component for ABA signal transduction of angiosperms. In contrast, subclass I SnRK2 emerged during evolution of vascular plants and is activated by osmostress but not by ABA. To get insights into the functional evolution of SnRK2 in land plants, we focus on the moss Physcomitrella patens, of which genome encodes only subclass III SnRK2 genes. We performed transcriptome analysis to investigate SnRK2-regulated genes in P. patens.