RNA-sequencing of Early vs Late Mouse Pancreas Reentrainment

The objectives of this study was to understand the implication of early (day 1:ZT8) vs late (day 8 [for female]/day 9 [for males]:ZT176/200) reentrainment in mouse pancreas following a chronic (1-month) phase shift protocol. 4-6 week old mice underwent 4 weeks of either a normal circadian light-dark cycle or a circadian disrupted light-dark cycle whereby mice were phase shifted forward 8 hours every 2-3 days. At 4 weeks all mice were normalized to normal circadian conditions 12 hours prior to the 4 week mark. Mice were then sacraficed at 14:00 each day. Of the mice evaluated, those from ZT8 and ZT176/200 were selected for further evaluation with RNA-sequencing. A total of 24 samples were included from two timepoints. At each timepoint, 6 mice were evaluated from each condition, including normal circadian and circadian disrupted conditions. A total of 3 males/females were included per condition per timepoint.