Efole et al. AJP-GI

Figure S1. Protein expression analysis by western blot with stain-free normalization. (A) Western blot of tyrosine hydroxylase (TH) expression in the liver from the full cohort of mice fed chow or GAN diet for 12, 20 or 32 weeks. Stain-free gel images are shown below and were used for normalization of protein expression. (B) Western blot of α-smooth muscle actin (α-SMA) levels in livers of WT and Adra1bLKO mice fed chow or GAN diet for 32 weeks, with corresponding stain-free gels used for normalization.Figure S2. Biochemical and molecular characterization of WT and Adra1bLKO mice after 12 weeks of GAN diet. A) Plasma alanine aminotransferase (ALT), (B) aspartate aminotransferase (AST), (C) total bilirubin, and (D) albumin levels in WT and Adra1bLKO after 12 weeks of GAN diet. (E) Relative hepatic mRNA expression of Tnf. (F) Relative hepatic mRNA expression of Il1b. (G) Relative hepatic mRNA expression of Il10. (H) Relative hepatic mRNA expression of Ccl2. (I) Relative hepatic mRNA expression of Adgre1. (J) Relative hepatic mRNA expression of Cd68. (K) Relative hepatic mRNA expression of Arg1. (L) Relative hepatic mRNA expression of Tgfb. (M) Relative hepatic mRNA expression of Acta2. (N) Relative hepatic mRNA expression of Timp1. Data are presented as mean ± SEM; n=8-9 mice per group.