RNA Sequencing Gene Expression of Endothelial differentiation in DMP1 treated DPSCs.

Regenerative vascular medicine has received considerable attention due to recent advancements suggesting that the conversion of somatic cells into endothelial cells offers a promising approach to address diseases related to abnormal vessel growth and endothelial cell dysfunction. The therapeutic potential of this method lies in the ability of DPSCs, upon exposure to DMP1, to differentiate into endothelial cells and support the formation of new blood vessels. Despite this progress, there is a need for a comprehensive understanding of the expression profile during endothelial differentiation. Gene ontology and genome functional enrichment analyses have provided insight into the differentiation of ECs from DMP1-treated DPSCs in conjunction with HUVEC-ECM, as compared to positive HUVECs. Our RNA sequence data underscores the striking similarity in gene expression patterns between the sorted DPSCs treated with DMP1 and the positive control HUVECs. These findings could contribute to a comprehensive understanding of the molecular mechanisms underlying the impact of DMP1 treatment on DPSCs, particularly in the context of guiding endothelial cells. We analyzed gene expression to examine endothelial-specific gene expression, cell morphogenesis, and tube development in sequencing data for sorted DPSCs treated with DMP1 and HUVECs. After 7 days culture, the cells were collected, rinsed with PBS, and then incubated with an endothelial specific antibodies CD31 and C144 under dark at ambient conditions for 40 minutes followed by flow cytometry sorting using a Moflo Astrios EQ device (UIC RRC facility). The pre-sorted and post-sorted DPSCs were used immediately for RNA Seq-Analysis.